In the original anime production of Sailor Moon, Ami is voiced by veteran voice actress Aya Hisakawa. After the show's conclusion, Hisakawa wrote in an artbook that she was "raised by" the character of Ami, and was "really, greatly happy" to have met her.
For the English-language dub, the voice of "Amy" is provided first by Karen Bernstein, for the original and R series and the movies, and later by Liza Balkan for the S and Supers series. Shandi Sinnamon also provides the English vocals for a song attributed to Amy's character in the second story arc.
Ami has been portrayed by 6 actresses in the stage musicals: Ayako Morino, Yukiko Miyagawa, Hisano Akamine, Mariya Izawa, Chieko Kawabe, and Manami Wakayama.
In the live-action series, she is played by Chisaki Hama. Child actress Kanki Matsumoto portrays Ami in flashback sequences and childhood photographs.