Miyū Sawai as Usagi Tsukino/Sailor Moon/Princess Serenity/Princess Sailor Moon/Queen Serenity
Chisaki Hama as Ami Mizuno/Sailor Mercury/Dark Mercury
Keiko Kitagawa as Rei Hino/Sailor Mars
Myū Azama as Makoto Kino/Sailor Jupiter
Ayaka Komatsu as Minako Aino/Sailor V/Sailor Venus
Rina Koike as Luna Tsukino/Sailor Luna
Keiko Han as Luna (voice, cat form)
Kappei Yamaguchi as Artemis (Sailor Moon) (voice)
Jyōji Shibue as Mamoru Chiba/Tuxedo Kamen/Prince Endymion
Aya Sugimoto as Queen Beryl
Jun Masuo as Jadeite (Sailor Moon)
Hiroyuki Matsumoto as Nephrite (Sailor Moon)
Yoshito Endō as Zoisite (Sailor Moon)
Akira Kubodera as Kunzite (Sailor Moon)
Alisa Durbrow as Mio Kuroki
Chieko Kawabe as Naru Osaka
Masaya Kikawada as Motoki Furuhata
Kaori Moriwaka as Ikuko Tsukino
Naoki Takeshi as Shingo Tsukino
Moeko Matsushita as Hina Kusaka
Narushi Ikeda as Sugao Saitou, Minako's manager
Many of these actors also appear in the Kamen Rider series, including Jyouji Shibue (Mamoru), as Kamen Rider Ibuki in Kamen Rider Hibiki (and later in Kamen Rider Decade as well), and Masaya Kikawada (Motoki), as Kamen Rider Ichigo (Kamen Rider: The First and Kamen Rider The Next). Hiroyuki Matsumoto (Nephrite) and Akira Kubodera (Kunzite) also appear, as well as Tomohisa Yuge (the fake Tuxedo Mask from PGSM Act 9), who was the suit actor for Kamen Rider Zolda (from Kamen Rider Ryuki) and Kamen Rider TheBee (from Kamen Rider Kabuto), Satoshi Ichijo (Yuuto from Act 32), who played Kamen Rider Gai in Kamen Rider Ryuki. More recently, Rina Koike (Luna in human form) played Shizuka Nomura from Kamen Rider Kiva, and Miyuu Sawai (Usagi) appeared in the Double: Begins Night segment of Kamen Rider × Kamen Rider Double & Decade: Movie War 2010 as Erika Mutsuki, a deceased musician whose ghost apparently had returned to haunt her sister.
Keiko Han and Chieko Kawabe had been involved with Sailor Moon previously: Keiko reprise their roles as Luna's (in cat form) voice in the anime and Chieko previously played Sailor Mercury in the Sailor Moon Musicals or SeraMyu.
The cloaked youma which appeared starting in ep. 37 are redesigned Golem Hei from the 1992 Super Sentai series Kyōryū Sentai Zyuranger (which were adapted to the Putty Patrol in Mighty Morphin Power Rangers).
Keiko Kitagawa (Rei) also made an appearance in The Fast and the Furious: Tokyo Drift, as Reiko.