Although Pretty Guardian Sailor Moon is a retelling of the first manga story arc, there are many differences that set it apart from the manga and anime. The storylines are more character-based and driven, focusing on the girls' civilian lives and their connection to the past more than on action sequences. While the first few episodes seemed directly based on anime and manga story lines, by the time Sailor Jupiter had arrived the show was starting to spin off in its own direction. Additionally, Usagi and Rei's relationship is closer in spirit to the manga; while they have their disagreements, it never gets to the heated level that it does in the anime.
One of the largest changes was to the character of Minako Aino, who, rather than being an ordinary girl among the other Senshi, is a famous pop idol. When introduced, she is fighting crime under the alias "Sailor V" (as in the other versions), and makes subtle reference to this double life in her music. Her most popular song, "C'est La Vie (Watashi no Naka no Koisuru Bubun)" (C'est la vie 〜私のなかの恋する部分), is a Japanese pun: "Sailor V" (セーラーV Sērā Bui) and "C'est la vie" (セ・ラ・ヴィ Se Ra Vi) are pronounced nearly identically.
In addition to plotline changes, some updating has been done to minor elements of the series, making them more in line with modern culture. For example, in the original anime and manga, there were scenes involving Ami and a cassette tape. In the new version, the tape is replaced by a MiniDisc. Instead of a transformation pen and communicators, each Senshi is given a magical camera phone and bracelets. Also, their secret hideout is not hidden in a video arcade, but rather in a magic karaoke room.
With the new adaptation of the show, certain characters were modified to give it freshness and originality. New aspects and forms include Sailor Luna, Dark Sailor Mercury, and Princess Sailor Moon. A new antagonist, Mio Kuroki, is also introduced.